The etiology of inflammatory bowel disease (IBD) is unknown, but it has become evident that genetic factors are involved in disease susceptibility.
Studies have suggested a north-south gradient in the incidence of inflammatory bowel disease.
This raises the question whether this difference is caused by genetic heterogeneity.
Dr Lene Riis and colleagues from Denmark investigated the prevalence of polymorphisms in CARD15 and TLR4 and occurrence of anti-Saccharomyces cerevisiae.
The researchers evaluated the antineutrophil cytoplasmic antibodies in a European population-based inflammatory bowel disease cohort.
The team genotyped individuals from the incident cohort for 3 mutations in CARD15 and 1 mutation in TLR4.
|TLR4 mutation rate was 8% in Crohn's patients|
|Inflammatory Bowel Disease|
Levels of anti-Saccharomyces cerevisiae and antineutrophil cytoplasmic antibodies were assessed.
The research team obtained disease location and behavior at time of diagnosis from patient files.
The research team found that the overall CARD15 mutation rate was 24% for Crohn's disease and 10% for ulcerative colitis patients.
Mutations were less present in the Scandinavian countries, occurring in 12%, versus 33% in the rest of Europe.
The researchers overall population attributable risk was 11%.
The TLR4 mutation rate was 8% in Crohn's disease patients, 7% in ulcerative colitis patients, and 12% in healthy controls.
The team noted that the TLR4 mutation rate was highest among South European Crohn's disease patients, and healthy controls.
The researchers found anti-Saccharomyces cerevisiae in 29% of Crohn's disease patients with no north-south difference.
The team identified that it was associated with complicated disease.
Antineutrophil cytoplasmic antibodies were most common in North European ulcerative colitis patients, and not associated with disease phenotype.
Dr Riis' team concluded, “The prevalence of mutations in CARD15 varied across Europe, and was not correlated to the incidence of Crohn's disease.
“There was no association between mutations in TLR4 and inflammatory bowel disease.”
“The prevalence of anti-Saccharomyces cerevisiae was relatively low, however, related to severe Crohn's disease."