Different protocols show that 13C-urea breath test is capable of assessing the intragastric Helicobacter pylori bacterial load.
However, others have not confirmed these data.
Prof Franco Bazzoli and colleagues from Italy to evaluate the correlation between 13C- urea breath test values and H pylori bacterial load.
The investigators enrolled 192 patients diagnosed H pylori-positive by rapid urease test, histology, and 13C- urea breath test.
|At 30 minutes, the excess delta 13CO2 excretion was 49 with a H pylori score of 3|
H pylori bacterial load and gastritis activity were evaluated according to the Updated Sydney System.
The investigative team performed the 13C- urea breath test according to the European Standard Protocol.
Breath samples were obtained every 10 minutes for 60 minutes in 52 patients and at 30 minutes in 140 patients and analyzed by mass spectrometry.
At 30 minutes, the mean excess delta 13CO2 excretion was 17, 30, and 49 in patients with H pylori scores 1, 2, and 3, respectively.
The investigators found that the difference was statistically significant.
The team also noted a significant positive correlation between H pylori score and activity score of chronic gastritis.
At 40 minutes and 50 minutes significant correlation between mean excess delta 13CO2 excretion was observed.
The investigators found that bacterial load was achieved only in patients with H pylori scores 1 and 3.
Dr Bazzoli's team concluded, “13C- urea breath test European Standard Protocol values correlate with H pylori bacterial load and the activity of gastritis at 30 minute breath sampling time.