Helicobacter pylori culture typically requires endoscopy.
Dr Graham and colleagues from Texas, America undertook a study in order to develop a minimally invasive rapid and reliable method to obtain H. pylori cultures.
The research team constructed an extendable oro-gastric brush, contained within a plastic over-tube (Baylor Brush, US Endoscopy).
After clinicians administered topical oral anesthesia, participants swallowed the 5-mm diameter brush assembly.
The investigators extended the brush in the stomach and brushed the mucosa 3 or 4 times.
The brush was then retracted into the protective sleeve and withdrawn from the patient.
|Helicobacter pylori recovery rate was 100% when cultured immediately|
The brush was either cultured directly or placed in cysteine transport medium with 20% glycerol which was then sampled immediately or after freezing at 70°C.
The researchers studied a total of 25 adult Helicobacter pylori-infected subjects (13 male, 12 female).
The research team found that Helicobacter pylori recovery rate was 100% (11 of 11) when cultured immediately.
The team also found recovery rate to be 100% after storage in transport medium at 70°C for 1 or 2 weeks or after storage at 4°C for 24 hours (four of four) or 72 hours (four of four) before being cultured.
Freezing on dry ice and air shipment did not reduce recovery.
Dr Graham concluded, "Rapid, reliable, nonendoscopic culture of gastric mucus is a practical method to obtain culture of Helicobacter pylori for clinical or research purposes."
"The method is amenable to being performed in a doctor's office or in the field."