Help
Subscribe


GastroHep.com - the global online resource for all aspects of gastroenterology, hepatology and endoscopy

 26 May 2018

Advanced search
GastroHep.com - the global online resource for all aspects of gastroenterology, hepatology and endoscopy Profile of Roy Pounder

Home

News  
Journals
Review Articles
Slide Atlas
Video Clips
Online Books
Advanced Digestive Endoscopy
Classical Cases
Conference Diary
PubMed
International GH Links
USA GH Links
National GH Links
National GI Societies
Other Useful Links




Emails on Gastroenterology and Hepatology
the National AIDS Treatment Advocacy Project
Visit the gastroenterology section of the EUMS

News

Role for mucosally adherent bacteria in Crohn's disease and colon cancer

Mucosally adherent bacteria may play a prominent role in the pathogenesis of Crohn's disease and colon cancer, suggests a report in the latest issue of Gastroenterology, which also highlights a potential role for soluble plantain fiber in Crohn's disease.

News image

fiogf49gjkf04

Inflammatory bowel disease and colon cancer is associated with altered mucosal glycosylation and it has been postulated that this could affect mucosal bacterial adhesion.

Therefore scientists from the University of Liverpool and the University of Southampton, UK, have aimed to characterize mucosa-associated and intramucosal bacteria, in particular Escherichia Coli.

Helen M Martin and colleagues isolated mucosa-associated bacteria, after dithiothreitol mucolysis from biopsy samples.

A total of 59 biopsy samples were obtained at colonoscopy from patients with either Crohn's disease (n = 14), ulcerative colitis (n = 21) or non-inflamed controls (n = 24).

A further 21 biopsy samples were collected at surgical resection from patients with colon cancer.

Presence of mucosa-associated E.coli:
Crohn's disease samples - 43%
Non-inflamed controls – 17%
Gastroenterology

As well as mucosa-associated bacteria, intramucosal bacteria were grown after gentamicin treatment followed by hypotonic lysis.

Both mucosa-associated and intramucosal bacteria were cultured more commonly in Crohn's disease samples (79% and 71% respectively) than in non-inflamed controls (42% and 29% respectively).

However, this was not the case with ulcerative colitis, where mucosa-associated and intramucosal bacteria were only cultured in 38% and 48% of samples, respectively.

Both bacteria were commonly cultured from colon cancers (71% and 57% respectively).

Mucosa-associated E. coli, which accounted for 53% of isolates, were more common in Crohn’s disease (6 of 14; 43%) than in non-inflamed controls (4 of 24, 17%).

The same was also true for intramucosal E. coli (Crohn’s disease, 29%; controls, 9%).

E. coli expressed hemagglutinins in 39% of Crohn’s cases and 38% of cancers but only 4% of controls, and this correlated with adherence to the I407 and HT29 cell lines, with invasion being cell-line dependent.

E. coli, including non-adherent isolates, induced interleukin-8 release from the cell lines. E. coli adhesins showed no blood group specificity, excepting 1 cancer isolate (HM44) with specificity for the Thomsen-Friedenreich antigen, but they could be blocked by soluble plantain fiber.

Commenting on their findings, the authors said, "These studies support a central role for mucosally adherent bacteria in the pathogenesis of Crohn’s disease and colon cancer."

They add that soluble plant fibers that inhibit their adherence have therapeutic potential, and hope to continue this work further, with the University of Liverpool having filed a patent application regarding the use of soluble plantain fiber in Crohn's disease, as a consequence of this work.

Gastroenterology 2004; 127 (1): 80
08 July 2004

Go to top of page Email this page Email this page to a colleague

 25 May 2018 
Colorectal cancer–specific mortality
 25 May 2018 
Technology to increase colorectal cancer screening
 25 May 2018 
Post‐operative complications in elderly IBD
 24 May 2018 
Patient-reported outcome measures in IBD trials
 24 May 2018 
Alcohol consumption and outcomes in drug-induced liver injury
 24 May 2018 
Precision medicine for tumors
 23 May 2018 
Management of perianal fistulas in Crohn’s disease
 23 May 2018 
Cardiovascular risk in diabetes mellitus with NAFLD
 23 May 2018 
High body mass index is and ulcerative colitis
 22 May 2018 
Worldwide H.pylori prevalence
 22 May 2018 
PPI and risk of stroke
 22 May 2018 
Online tool predicts bowel dysfunction severity prior to anterior resection
 21 May 2018 
Depressive symptoms in IBD youth
 21 May 2018 
PPI use and cognitive decline
 21 May 2018 
Fecal incontinence and quality of life in IBD
 18 May 2018 
Esophageal dilatation in clinical practice 
 17 May 2018 
IBD and later extraintestinal manifestations
 17 May 2018 
Repeat stool DNA testing
 17 May 2018 
IBS and chronic fatigue following GI infection
 16 May 2018 
Factors associated with fecal incontinence
 16 May 2018 
Diagnostic delay in Crohn's disease
 16 May 2018 
Cardiovascular risk in diabetes mellitus with NAFLD
 15 May 2018 
Guidelines for management of Crohn's
 15 May 2018 
Hep B in the Grey Zone
 15 May 2018 
New therapies for CDI
 14 May 2018 
Blood test for the diagnosis of fibrotic NASH
 14 May 2018 
Outcomes at bariatric centers of excellence
 14 May 2018 
Management of perianal fistulas in Crohn’s
 11 May 2018 
Detection of undiagnosed celiac disease
 11 May 2018 
Alcohol consumption and drug-induced liver injury
 10 May 2018 
Colorectal cancer screening
 10 May 2018 
Fibrosis in patients with chronic hepatitis B
 09 May 2018 
Fecal incontinence
 09 May 2018 
Health problems and IBS
 09 May 2018 
Esophageal dilatation in clinical practice 
 07 May 2018 
Omitting antibiotics in uncomplicated acute diverticulitis
 07 May 2018 
Health problems and IBS
 07 May 2018 
Assessment of diminutive colorectal polyps
 04 May 2018 
National Institutes of Health workshop and obesity
 04 May 2018 
Factors associated with fecal incontinence
 04 May 2018 
Colorectal cancer screening and ethnic inequities
 03 May 2018 
Gastrointestinal ultrasound in IBD
 03 May 2018 
Ultransonography in postsurgical recurrence in Crohn's
 02 May 2018 
Symptom assessment in cirrhotic ascites
 02 May 2018 
Hep C antiviral treatment and liver cancer risk
 02 May 2018 
Chronic Hep B
 01 May 2018 
European guidelines on pancreatic cystic neoplasms
 01 May 2018 
Interferon‐free regimens in Hep C
 01 May 2018 
Age at IBD onset and extraintestinal complications
 30 April 2018 
Formula for preterm infants
 30 April 2018 
Primary antibiotic resistance and sequential therapy for H. pylori
 27 April 2018 
Eastern and western practices for endoscopic resection of colorectal lesions
 27 April 2018 
Anxiety scale for chronic esophageal disease
 27 April 2018 
Disease activity in eosinophilic esophagitis
 26 April 2018 
Obesity and physical activity in diverticulosis
 26 April 2018 
Primary sclerosing cholangitis in the USA
 25 April 2018 
Aspirin and pancreatic cancer
 25 April 2018 
Central mediators of irinotecan-induced steatohepatitis
 25 April 2018 
Neuroimaging in Hep C
 24 April 2018 
Scoring system identifies cancer risk in Barrett's

Blackwell Publishing


GastroHep.com is a Blackwell Publishing registered trademark
© 2018 Wiley-Blackwell and GastroHep.com and contributors
Privacy Statement
Disclaimer
About Us