Several gene mutations are involved in the carcinogenesis of colorectal adenomas and carcinomas.
Of these gene mutations, K-ras and p53 are thought to be of particular importance.
Dr M Heinzlmann and colleagues investigated whether these mutations can be detected in the colonic lavage fluid from patients with colorectal adenomas and carcinomas.
The research team analyzed the colonic lavage fluid of 45 patients with adenomas, 20 patients with colorectal carcinomas, and 38 patients with nonneoplastic and noninflammatory diseases of the colon.
|K-ras and p53 mutations found in lavage fluid of 25% of patients with carcinoma|
K-ras and p53 mutations were detected using single-strand conformation polymorphism and dot-blot hybridization.
Mutations of the K-ras and p53 genes were found in the colonic lavage fluid of 15.6% of patients with adenomas, 25.0% of patients with carcinomas and 2.6% of the control group.
Dr Heinzlmann concludes, "Genetic alterations in colonic lavage fluid could be used in the diagnosis of patients with colorectal neoplasias."