The researchers assessed the effectiveness of 1H magnetic resonance spectroscopy (MRS) in differentiating ulcerative colitis from Crohn's disease.
Their findings have been reported in the February issue of the American Journal of Gastroenterology.
1H MRS assessed colonic biopsies (45 ulcerative colitis and 31 Crohn's disease), and multivariate analysis was applied to distinguish the two diseases.
A second study was performed on biopsies taken from patients with a history of inflammatory bowel disease (IBD), but with normal mucosa endoscopically and histologically.
|Classification accuracy of 1H magnetic resonance spectroscopy was 98%.|
|American Journal of Gastroenterology|
A method of classification was developed using the spectra from 76 inflamed IBD and 25 control tissues.
The spectra of 38 biopsies obtained from endoscopically and histologically normal areas of the colons of patients with IBD were put into the validation test set.
The classification accuracy between ulcerative colitis and Crohn's disease was found to be 98.6%, with only one case of Crohn's disease and no cases of ulcerative colitis being misclassified.
The diagnostic spectral regions identified by the researchers' algorithm included those for taurine, lysine, and lipid.
In the second study, the classification accuracy between normal controls and IBD was 97.9%.
Only 47.4% of the endoscopically and histologically normal IBD tissue spectra were classified as true normals; 34.2% showed 'abnormal' magnetic resonance spectral profiles, and the remaining 18.4% could not be classified unambiguously.
Dr T. Bezabeh concluded on behalf of the group, "There is a strong potential for MRS to be used in the accurate diagnosis of indeterminate colitis; it may also be sensitive in detecting preclinical inflammatory changes in the colon."