Dr Gustafsson and colleagues conducted a study to elucidate the mechanisms of development of cholesterol crystals and gallstones during weight reduction in obese subjects.
The researchers included 25 morbidly obese, gallstone-free subjects, who underwent vertical-banded gastroplasty.
The research team collected gallbladder bile at the time of the operation via needle aspiration.
|In patients with less than 2 months follow-up, cholesterol saturation increased from 90% to 114%|
Gallbladder bile was also collected by the team at 1 to 7 months after the operation via ultrasound-guided transhepatic puncture of the gallbladder.
The researchers reported that during the follow-up time, the mean weight loss was 17 kg.
The team found that 2 patients developed gallstones and 10 patients displayed cholesterol crystals in their bile.
In 13 patients with a follow-up time of less than 2 months, cholesterol saturation increased from 90% to 114%.
The researchers noted that cholesterol saturation tended to decrease in the patients with a follow-up time of more than 2 months.
The extraction of the concanavalin-A-binding fraction from gallbladder bile was obtained after weight reduction in 7 patients.
The researchers observed that concanavalin-A-binding fraction prolonged crystallization detection time from 6 to 10 days.
In addition, the team found hexosamine concentration, a marker for mucin, increased by 100% in bile obtained in 6 of 7 patients after weight reduction.
Dr Gustafsson's team concludes, “The results indicate that crystallization-promoting compounds (mucin) are of great importance in the development of cholesterol crystals and gallstones in obese subjects during weight reduction, probably because of defective gallbladder emptying.”