Dr Kaplanskiab and colleagues from France aimed to better characterize the molecules involved in leukocyte tissue infiltration during Hepatitis C-mixed cryoglobulinemia associated vasculitis.
The researchers evaluated the involvement of endothelial cell leukocyte adhesion molecule-1, intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1.
The team included 36 patients with Hepatitis C- mixed cryoglobulinemia associated vasculitis.
The investigative team used 3 different approaches including ELISA analysis, and tissue expression by immunohistochemistry on patients nerve biopsies.
| There are elevated concentrations of soluble vascular adhesion molecule-in patients with Hepatitis C mixed cryoglobulinemia vasculitis|
|Journal of Hepatology|
The investigators also used endothelial expression by analysis on cells activated in vitro by cryoprecipitates purified from patients with Hepatitis C mixed cryoglobulinemia associated vasculitis.
The researchers found elevated concentrations of soluble vascular adhesion molecule-in patients with Hepatitis C mixed cryoglobulinemia vasculitis compared to Hepatitis C vasculitis patients without mixed cryoglobulinemia, with the highest concentrations in severe vasculitis.
The researchers detected vascular cell adhesion molecule-1 expression on blood vessels from nerve biopsies performed in patients with severe vasculitis.
The investigators found that cryoprecipitate induced vascular cell adhesion molecule-1, endothelial cell leukocyte adhesion molecule-1 and intercellular cell adhesion molecule-1 expression in patients with Hepatitis C mixed cryoglobulinemia vasculitis when added to endothelial cells in vitro.
The team thought this was possibly through a mechanism due to the C1q complement fraction interaction with endothelial cells.
Dr Kaplanskiab reports that, “Vascular cell adhesion molecule-1 is mainly involved in the pathogenesis of Hepatitis C mixed cryoglobulinemia vasculitis associated severe vasculitis and may be a potential interesting therapeutic target.”