Help
Subscribe


GastroHep.com - the global online resource for all aspects of gastroenterology, hepatology and endoscopy

 23 February 2018

Advanced search
GastroHep.com - the global online resource for all aspects of gastroenterology, hepatology and endoscopy Profile of Roy Pounder

Home

News  
Journals
Review Articles
Slide Atlas
Video Clips
Online Books
Advanced Digestive Endoscopy
Classical Cases
Conference Diary
PubMed
International GH Links
USA GH Links
National GH Links
National GI Societies
Other Useful Links




Emails on Gastroenterology and Hepatology
the National AIDS Treatment Advocacy Project
Visit the gastroenterology section of the EUMS

News

Role for mucosally adherent bacteria in Crohn's disease and colon cancer

Mucosally adherent bacteria may play a prominent role in the pathogenesis of Crohn's disease and colon cancer, suggests a report in the latest issue of Gastroenterology, which also highlights a potential role for soluble plantain fiber in Crohn's disease.

News image

fiogf49gjkf04

Inflammatory bowel disease and colon cancer is associated with altered mucosal glycosylation and it has been postulated that this could affect mucosal bacterial adhesion.

Therefore scientists from the University of Liverpool and the University of Southampton, UK, have aimed to characterize mucosa-associated and intramucosal bacteria, in particular Escherichia Coli.

Helen M Martin and colleagues isolated mucosa-associated bacteria, after dithiothreitol mucolysis from biopsy samples.

A total of 59 biopsy samples were obtained at colonoscopy from patients with either Crohn's disease (n = 14), ulcerative colitis (n = 21) or non-inflamed controls (n = 24).

A further 21 biopsy samples were collected at surgical resection from patients with colon cancer.

Presence of mucosa-associated E.coli:
Crohn's disease samples - 43%
Non-inflamed controls – 17%
Gastroenterology

As well as mucosa-associated bacteria, intramucosal bacteria were grown after gentamicin treatment followed by hypotonic lysis.

Both mucosa-associated and intramucosal bacteria were cultured more commonly in Crohn's disease samples (79% and 71% respectively) than in non-inflamed controls (42% and 29% respectively).

However, this was not the case with ulcerative colitis, where mucosa-associated and intramucosal bacteria were only cultured in 38% and 48% of samples, respectively.

Both bacteria were commonly cultured from colon cancers (71% and 57% respectively).

Mucosa-associated E. coli, which accounted for 53% of isolates, were more common in Crohn’s disease (6 of 14; 43%) than in non-inflamed controls (4 of 24, 17%).

The same was also true for intramucosal E. coli (Crohn’s disease, 29%; controls, 9%).

E. coli expressed hemagglutinins in 39% of Crohn’s cases and 38% of cancers but only 4% of controls, and this correlated with adherence to the I407 and HT29 cell lines, with invasion being cell-line dependent.

E. coli, including non-adherent isolates, induced interleukin-8 release from the cell lines. E. coli adhesins showed no blood group specificity, excepting 1 cancer isolate (HM44) with specificity for the Thomsen-Friedenreich antigen, but they could be blocked by soluble plantain fiber.

Commenting on their findings, the authors said, "These studies support a central role for mucosally adherent bacteria in the pathogenesis of Crohn’s disease and colon cancer."

They add that soluble plant fibers that inhibit their adherence have therapeutic potential, and hope to continue this work further, with the University of Liverpool having filed a patent application regarding the use of soluble plantain fiber in Crohn's disease, as a consequence of this work.

Gastroenterology 2004; 127 (1): 80
08 July 2004

Go to top of page Email this page Email this page to a colleague

 23 February 2018 
Patients on antithrombotic agents undergoing emergency and elective endoscopy
 23 February 2018 
Heavy metals on a gluten-free diet
 23 February 2018 
MRI and NAFLD
 22 February 2018 
Outcomes with Crohn’s after infliximab withdrawal
 22 February 2018 
Elderly onset of IBD

 22 February 2018 
Autophagy enhancers
 21 February 2018 
Management of hemorrhoids in the USA
 21 February 2018 
Adalimumab and infliximab in biologic-naïve Crohn's
 21 February 2018 
Cystic fibrosis and colorectal cancer
 20 February 2018 
Complications and surveillance colonoscopies
 20 February 2018 
Treatment algorithm for polyp cancers
 20 February 2018 
Predictors of postoperative infection in Crohn's
 19 February 2018 
Screening colonoscopy in the right and left colon
 19 February 2018 
NAFLD prevalence in the USA
 19 February 2018 
Fructans in children with IBS

 16 February 2018 
Inflammatory bowel diseases are global diseases
 16 February 2018 
Undetected celiac in the elderly
 16 February 2018 
Fructans induce non-celiac gluten sensitivity
 15 February 2018 
NSAIDS and GI damage
 15 February 2018 
Oral direct-acting antiviral treatment for Hep C virus genotype 1
 15 February 2018 
Primary vs secondary surgery for the presence of lymph node metastasis
 14 February 2018 
Predicting adenoma detection rate
 14 February 2018 
Normal bowel frequency characterization in the USA 
 13 February 2018 
Personalising treatment options for IBS
 13 February 2018 
Prebiotics improve endothelial dysfunction
 13 February 2018 
Diagnostic criteria for a Rome IV functional gastrointestinal disorders
 12 February 2018 
Visceral hypersensitivity and functional GI disorders
 12 February 2018 
Depression and aggressive IBD
 12 February 2018 
Variability in interpretation of endoscopic findings impacts patient management
 09 February 2018 
Treatment of choice for anastomotic stricture in IBD
 09 February 2018 
PRO measurement information system 
 09 February 2018 
Overall disease severity indices for IBD
 08 February 2018 
Prediction of endoscopically active disease

 08 February 2018 
Steroid-refractory acute severe ulcerative colitis
 08 February 2018 
Decision aid used by IBD patients
 07 February 2018 
Ursodeoxycholic acid combined with bezafibrate for itching
 07 February 2018 
Change in microbiome in gastritis vs gastric carcinoma
 07 February 2018 
Colorectal cancer and primary sclerosing cholangitis-IBD
 06 February 2018 
Risk of death after liver transplantation
 06 February 2018 
Crohn’s disease vs refractory pouchitis
 06 February 2018 
Support for functional dyspepsia symptom diary
 05 February 2018 
Helicobacter spp influence on GI tract 
 05 February 2018 
No link found between severe reflux and all-cause mortality 
 05 February 2018 
Psychological distress in PPI non-responders
 02 February 2018 
Assessing psychosexual impact of IBD
 02 February 2018 
Decrease in overall mortality with cholera vaccination
 02 February 2018 
Diagnostic performance of fecal immunochemical tests
 01 February 2018 
Screening frequency with family histories of colorectal cancer
 01 February 2018 
IBD and sport participation
 01 February 2018 
Life with a stoma 
 31 January 2018 
Aprepitant and gastroparesis 
 31 January 2018 
Anesthesia risk in colonoscopy
 31 January 2018 
GED-0301 for Crohn's Disease
 30 January 2018 
Intestinal dysbiosis and allergic diseases in infants
 30 January 2018 
Fructans and IBS symptoms in children
 29 January 2018 
Dosing calculator for therapy optimization in IBD
 29 January 2018 
Glecaprevir–pibrentasvir for in HCV
 29 January 2018 
Food allergen injections in eosinophilic esophagitis
 29 January 2018 
Reliability of the IBD index
 26 January 2018 
Tofacitinib vs biological therapies for ulcerative colitis

Blackwell Publishing


GastroHep.com is a Blackwell Publishing registered trademark
© 2018 Wiley-Blackwell and GastroHep.com and contributors
Privacy Statement
Disclaimer
About Us