In this study, a team of researchers from Spain evaluated 2 immunochromatographic visual assays as an alternative to enzyme-linked immunosorbent assays (ELISAs) for celiac disease screening.
The team used a tissue-transglutaminase stick to detect immunoglobulin A/G (IgA/G) antibodies to t-TG, and a t-TG/antigliadin antibodies (AGA) stick to detect IgA antibodies to both t-TG and AGA. They also used t-TG and AGA ELISAs.
Overall, the team assayed a total of 142 children and 30 adults, and 140 controls. They also assayed 23 sera from pediatric celiac patients in remission.
|Results were comparable with the corresponding ELISAs.|
|Clinical Gastroenterology and Hepatology|
The researchers found that the t-TG stick had a sensitivity of 97% and a specificity of 99% in pediatric patients; sensitivity was 83% and specificity 100% in adults.
In addition, they found that the t-TG/AGA stick had a sensitivity of 96% and a specificity of 99% for t-TG and a sensitivity of 89% and a specificity of 96% for AGA in children.
In adults, sensitivity was 80% and specificity 100% for t-TG, while sensitivity was 83% and specificity of 100% for AGA.
The research team found that results were comparable with the corresponding ELISAs.
Dr Sergio Ferre-lópez's team concluded, "The 2 visual assays are efficient for celiac disease screening as an alternative to ELISAs".
"They are simple to handle and to interpret".
"By the combined use of the 2 sticks, IgA-deficient patients can be identified as positive in the t-TG (IgG/A) but negative in the t-TG/AGA (IgA) stick".