The role of Helicobacter pylori and aspirin in peptic ulcer formation and recurrence remains an important clinical topic. However, the interaction between aspirin and H. pylori is not clear.
In this study, researchers from China and the United States investigated the effect of aspirin on the growth of H. pylori. They also assessed the effect of aspirin on the susceptibility of H. pylori to antimicrobials.
The team performed time killing studies of H. pylori using different concentrations of aspirin and salicylate.
They assessed bacteria growth spectrophotometrically and by viable colony count.
|Aspirin inhibited the growth of H pylori in a dose dependent manner.|
The effects of aspirin on the efficiency of colony formation and on metronidazole induced mutation to rifampicin resistance were also determined.
The team tested minimal inhibitory concentrations (MICs) of aspirin and metronidazole using the standard agar dilution method. MICs of amoxycillin and clarithromycin were determined by the E test method.
The researchers found that aspirin and salicylate inhibited the growth of H pylori in a dose dependent manner. Bactericidal activity was due to cell lysis.
Aspirin 400 µg/ml caused a 2 logs decrease in colony forming units/ml at 48 hours. It also suppressed the normal ability of metronidazole to induce new mutations to rifampicin.
The IC90 of aspirin was 512 µg/ml.
In addition, increased susceptibility to amoxycillin, clarithromycin, and metronidazole of H. pylori was observed at 1 mM (180 µg/ml) aspirin.
Dr Wang’s team concluded, “Aspirin inhibited the growth of H. pylori, suppressed the mutagenic effect of metronidazole, and enhanced the susceptibility of H. pylori to antimicrobial agents”.
“This mechanism is important in future drug development for effective clearing and overcoming resistance.”