A high incidence of Helicobacter pylori among the family members of children with H. pylori gastritis has been documented.
In this study, researchers from Greece sought genetic clarification of H. pylori strains involved in intrafamilial dispersion.
|Closely related H. pylori strains involved in intrafamilial dispersion.|
The research team studied formalin-fixed, paraffin-embedded material of antral mucosa from 32 members of 11 families. The team looked for the presence of genetic homogeneity in these samples.
To achieve this, the researchers studied the entire genome of H. pylori using polymerase chain reaction (PCR)-based random amplified polymorphic DNA (RAPD) fingerprinting.
In addition, they analyzed the Urease A gene using a multiplex PCR-assay, and an alternative mutation detection method.
The researchers determined that closely related H. pylori strains were involved in the intrafamilial dispersion.
Mutations and small deletions in Urease A gene were found in 22 individuals.
Dr Eleftheria Roma-Giannikou's team concluded, "The homology of the H. pylori genome in members of the same family strongly supports the hypothesis of transmission of H. pylori from person-to-person or from a common source".