Colonic microflora is involved in the pathogenesis of Crohn's disease (CD).
Less than 30% of this microflora is able to be cultured.
In this study, researchers from France investigated the differences in fecal microflora between patients with colonic CD in remission, active colonic CD, and healthy volunteers using culture independent techniques.
They measured the proportions of rRNA corresponding to each phylogenetic group using quantitative dot blot hybridization with 6 radiolabelled 16S ribosomal ribonucleic acid (rRNA) targeting oligonucleotide probes.
Temporal temperature gradient gel electrophoresis (TTGE) of 16S rDNA was used to evaluate dominant species diversity.
|Enterobacteria were significantly increased in active and quiescent Crohn's disease.|
The research team found that enterobacteria were significantly increased in active and quiescent CD.
In addition, probe additivity was significantly lower in active and quiescent CD, than in healthy controls.
The team also found that TTGE profiles varied markedly between active and quiescent CD, but were stable in healthy conditions.
Dr Seksik's team concluded, "The biodiversity of the microflora remains high in patients with CD".
"Enterobacteria were observed significantly more frequently in CD than in health, and more than 30% of the dominant flora belonged to yet undefined phylogenetic groups".