In this study, a team from the Netherlands explored the possible relation between Helicobacter spp. and gallbladder disorders.
The team sampled bile from 31 Dutch patients with biliary obstruction, identified by jaundice and dilatation of the bile ducts on ultrasound.
They obtained samples immediately following cannulation of the common bile duct (CBD) by endoscopic retrograde cholangiopancreatography (ERCP) in 29 patients, and by peri-operative puncture of the gallbladder in 2.
DNA was isolated from bile by binding to diatoms.
In addition, Helicobacter spp. were detected by a sensitive (detection limit 1 CFU per reaction tube) 16S rDNA PCR with genus-specific primers.
|H. pylori 16S rDNA found in only 1 out of 21 amplifiable bile samples.|
Furthermore, duplicate samples were spiked with Helicobacter pylori DNA and subjected to PCR in order to check for inhibition.
The research team found 28 patients had CBD stones, 2 had a pancreatic head tumor, and in 1 had no abnormalities.
The team found H. pylori 16S rDNA in only 1 out of 21 amplifiable bile samples (10 out of 31 were inhibited) from Dutch patients with CBD stones.
Dr Robert Roosendaala's team concluded, "Our data indicate that CBD stones in Dutch patients are not associated with the presence of Helicobacter spp. in bile".