A team from Paris and Nantes, France, used real-time RT-PCR to quantify viremia in hepatitis A virus (HAV) infection.
The duration of HAV presence and levels during the phases of clinical disease and convalescence has not yet been well studied in human patients.
The researchers optimized a one-step RT-PCR that contained a dual-labeled fluorogenic probe to quantify the 5' noncoding region (5'NCR) of HAV.
The coefficient of regression of the standard curve was, on average, 0.978.
Intra-assay coefficients of variation (CVs) varied from 6.1% to 0.98%, and interassay CVs from 6.5% to 2.1%.
| Viremia persisted for 60 days after HAV clinical onset.
| Journal of Viral Hepatitis |
A total of 41 HAV IgM-positive serum samples and 200 serum samples from healthy blood donors were tested by the quantitative RT-PCR method.
The mean value on the first day of diagnosis found was 6.38 x 105 copies/ml.
In a longitudinal study, viremia persisted for an average of 60 days after clinical onset.
M. Costa-Mattioli, of Central Regional Hospital of the University of Nantes, concluded on behalf of the group, "These results show that viremia in HAV infection lasts for many weeks."