Help
Subscribe


All of GastroHep is now free access! - Click here to register Read For FREE - Our full range of review articles
GastroHep.com - the global online resource for all aspects of gastroenterology, hepatology and endoscopy
GastroHep.com - the global online resource for all aspects of gastroenterology, hepatology and endoscopy Profile of Guido Tytgat Profile of Pete Peterson Profile of Peter Cotton Profile of Roy Pounder

Home

News  
Journals
Review Articles
Slide Atlas
Video Clips
Online Books
Advanced Digestive Endoscopy
Classical Cases
Conference Diary
PubMed
International GH Links
USA GH Links
National GH Links
National GI Societies
Other Useful Links




Emails on Gastroenterology and Hepatology
the National AIDS Treatment Advocacy Project
Visit the gastroenterology section of the EUMS

News

Lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes

This month's issue of Gastroenterology investigates integrated metabolite and gene expression profiles that identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

News image

Dr Anuradha Budhu from Maryland, USA combined gene expression and metabolic profiling analyses to identify factors associated with outcomes of patients with hepatocellular carcinoma.

The research team compared metabolic and gene expression patterns between paired tumor and nontumor tissues from 30 patients with hepatocellular carcinoma, and validated the results using samples from 356 patients with hepatocellular carcinoma.

The team reported that a total of 469 metabolites were measured using liquid chromatography/mass spectrometry and gas chromatography/mass spectrometry.

Metabolic and genomic data were integrated, and Kaplan–Meier and Cox proportional hazards analyses were used to associate specific patterns with patient outcomes.

The researchers found that the associated factors were evaluated for their effects on cancer cells in vitro and tumor formation in nude mice.

The research team identified 28 metabolites, and 169 genes associated with aggressive hepatocellular carcinoma.

The team of doctors noted that lipid metabolites of stearoyl-CoA-desaturase activity were associated with aberrant palmitate signaling in aggressive hepatocellular carcinoma samples.

Lipid metabolites of stearoyl-CoA-desaturase activity were associated with aberrant palmitate signaling
Gastroenterology

Expression of gene products associated with these metabolites, including stearoyl-CoA-desaturase, were associated independently with survival times and tumor recurrence in the test and validation sets.

The research team assessed that combined expression of stearoyl-CoA-desaturase and α-fetoprotein were associated with outcomes of patients with early-stage hepatocellular carcinoma.

Levels of monounsaturated palmitic acid, the product of stearoyl-CoA-desaturase activity, were increased in aggressive hepatocellular carcinoma.

The team found that monounsaturated palmitic acid increased migration and invasion of cultured hepatocellular carcinoma cells, and colony formation by hepatocellular carcinoma cells.

The team of doctors noted that hepatocellular carcinoma cells that expressed small interfering RNA against stearoyl-CoA-desaturase had decreased cell migration and colony formation in culture and reduced tumorigenicity in mice.

Dr Budhu's team concluded, "By using a combination of gene expression and metabolic profile analysis, the identified a lipogenic network that involves stearoyl-CoA-desaturase and palmitate signaling, and was associated with hepatocellular carcinoma progression and patient outcomes."

"The microarray platform and data have been submitted to the Gene Expression Omnibus public database at NCBI following MIAME guidelines."

Gastroenterology 2013: (144)5: 1066-1075
16 May 2013

Go to top of page Email this page Email this page to a colleague

 30 May 2015

Advanced search
 29 May 2015 
Comfort during colonoscopy
 29 May 2015 
Colorectal neoplasia in liver transplant candidates
 29 May 2015 
Cervical cancer with immunosuppressive therapy in IBD
 28 May 2015 
Excercise and IBD
 28 May 2015 
Probiotics and Crohn's disease
 28 May 2015 
Bleeding and colorectal endoscopic submucosal dissection
 27 May 2015 
Vegetables and fruit and IBD
 27 May 2015 
Radiofrequency ablation for treatment of GERD
 27 May 2015 
Management of constipation and IBS
 26 May 2015 
Antimicrobials for intraabdominal infection
 26 May 2015 
Calcium channel blockers and GI bleeds
 26 May 2015 

Risk factors for celiac disease

 25 May 2015 
Colorectal cancer screening
 25 May 2015 
Saccharomyces boulardii and H. pylori
 25 May 2015 
Risk of GI bleeds with NSAIDs
 22 May 2015 
Colonoscopy screening for colorectal cancer
 22 May 2015 
Treatment with anti-integrin antibodies in IBD
 22 May 2015 
Care of patients with GERD
 21 May 2015 
Lymphoma in IBD
 21 May 2015 
Serrated polyps and colorectal cancer
 21 May 2015 
Imaging for hepatocellular carcinoma
 20 May 2015 
Pancreatic neuroendocrine tumors
 20 May 2015 
Surveillance in long-segment Barrett's
 20 May 2015 
Refractory metastatic colorectal cancer
 19 May 2015 
Metabolic syndrome and Barrett's
 19 May 2015 
PPIs and cryptogenic liver abscess
 19 May 2015 
Anti-integrin antibodies in IBD
 18 May 2015 
ESPGHAN celiac guidelines
 18 May 2015 
HLA haplotypes and primary sclerosing cholangitis
 18 May 2015 
Safety of PPI
 15 May 2015 
Microscopic colitis risk with PPIs/NSAIDs
 15 May 2015 
Management of nonhospitalized ulcerative colitis
 15 May 2015 
Diabetes and infections in IBD with immunomodulation
 14 May 2015 
Diagnosis of spontaneous bacterial peritonitis
 14 May 2015 
Mortality in US patients with cirrhosis
 14 May 2015 
Radiation exposure during ERCP
 13 May 2015 
Predictors of IBD
 13 May 2015 
Psychological distress and liver disease mortality
 13 May 2015 
Hypnotherapy in IBS/IBD
 12 May 2015 
Bleeding in NSAID users with H.pylori
 12 May 2015 
Capsule colonoscopy detects colorectal polyps
 12 May 2015 
Anti-TNF biologics in acute severe ulcerative colitis
 11 May 2015 
Management of Barrett's esophagus
 11 May 2015 
Marker to detect recurrence of Crohn's
 11 May 2015 
Thalidomide for IBD
 08 May 2015 
Surveillance colonoscopy in colorectal cancer
 08 May 2015 
HCV treatment with compensated cirrhosis
 08 May 2015 
Medical management of Crohn's
 07 May 2015 
Surgical complications in ulcerative colitis
 07 May 2015 
Combination therapy for noncirrhostic patients
 07 May 2015 
Anxiety and new-onset dyspepsia
 06 May 2015 
PPIs and spontaneous bacterial peritonitis
 06 May 2015 
Infant feeding and celiac disease
 06 May 2015 
Preventing recurrent C. diff infection
 05 May 2015 
Fatigue in primary sclerosing cholangitis
 05 May 2015 
Annual CT scans after esophagectomy for cancer
 05 May 2015 
Prevalence of eosinophilic esophagitis
 04 May 2015 
Stress resilience and peptic ulcer disease
 04 May 2015 
Endoscope storage and microbial colonization
 04 May 2015 
Menarche and NAFLD

Blackwell Publishing


GastroHep.com is a Blackwell Publishing registered trademark
© 2015 Wiley-Blackwell and GastroHep.com and contributors
Privacy Statement
Disclaimer
About Us